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Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software

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Golgi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2557))

Abstract

The architecture of the Golgi apparatus in mammalian cells changes dynamically in response to internal and external cues and may be permanently altered in disease states. Here, we present a method to quantify changes in Golgi morphology using immunofluorescence and confocal microscopy followed by CellProfiler software analysis. This method will assist researchers in evaluating alterations in the Golgi complex morphology of cultured cells under a variety of different experimental conditions.

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Acknowledgement

We thank Sofia Geroyska for her critical comments on the manuscript.

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Division of Medical Hematology and Oncology, The Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA

    Isabel Mejia, Yu-Chuan Chen & Begoña Díaz

  2. David Geffen School of Medicine and Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Los Angeles, CA, USA

    Begoña Díaz

Authors
  1. Isabel Mejia
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  2. Yu-Chuan Chen
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  3. Begoña Díaz
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Corresponding author

Correspondence to Begoña Díaz .

Editor information

Editors and Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA

    Yanzhuang Wang

  2. Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA

    Vladimir V Lupashin

  3. Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA

    Todd R. Graham

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mejia, I., Chen, YC., Díaz, B. (2023). Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software. In: Wang, Y., Lupashin, V.V., Graham, T.R. (eds) Golgi. Methods in Molecular Biology, vol 2557. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2639-9_46

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  • DOI: https://doi.org/10.1007/978-1-0716-2639-9_46

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2638-2

  • Online ISBN: 978-1-0716-2639-9

  • eBook Packages: Springer Protocols

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