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Analysis of Golgi Protein Acetylation Using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry

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Golgi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2557))

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Abstract

Acetylation is one of the most abundant post-translational protein modifications that regulates all cellular compartments ranging from chromatin to cytoskeleton and Golgi. The dynamic acetylation of the Golgi stacking protein GRASP55 was shown to regulate Golgi reassembly after mitosis. Here we provide a detailed protocol for the analysis of Golgi acetylation including in vitro assays to detect protein acetylation and mass spectrometry analysis to identify specific acetylation sites and their relative abundance.

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Author information

Authors and Affiliations

  1. Department of Medical Biochemistry, Max Perutz Labs, Vienna Biocenter, Vienna, Austria

    Dea Slade

  2. Department of Radiation Oncology, Medical University of Vienna, Vienna, Austria

    Dea Slade

  3. Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria

    Dea Slade

  4. Mass Spectrometry Facility, Max Perutz Labs, University of Vienna, Vienna Biocenter, Vienna, Austria

    Markus Hartl

  5. Department of Biochemistry and Cell Biology, Max Perutz Labs, University of Vienna, Vienna Biocenter, Vienna, Austria

    Markus Hartl

Authors
  1. Dea Slade
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  2. Markus Hartl
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Corresponding authors

Correspondence to Dea Slade or Markus Hartl .

Editor information

Editors and Affiliations

  1. Department of Molecular, Cellular and Developmental Biology, University of Michigan, Ann Arbor, MI, USA

    Yanzhuang Wang

  2. Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR, USA

    Vladimir V Lupashin

  3. Department of Biological Sciences, Vanderbilt University, Nashville, TN, USA

    Todd R. Graham

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© 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Slade, D., Hartl, M. (2023). Analysis of Golgi Protein Acetylation Using In Vitro Assays and Parallel Reaction Monitoring Mass Spectrometry. In: Wang, Y., Lupashin, V.V., Graham, T.R. (eds) Golgi. Methods in Molecular Biology, vol 2557. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2639-9_43

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  • DOI: https://doi.org/10.1007/978-1-0716-2639-9_43

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2638-2

  • Online ISBN: 978-1-0716-2639-9

  • eBook Packages: Springer Protocols

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