Abstract
The roles of small molecules in every aspect of life have been gaining increased recognition. Many are known to exert their effect by binding proteins—but a comprehensive overview of protein–metabolite interactions (PMIs) is missing. Recently we devised a non-targeted method for detecting PMIs using size-exclusion chromatography followed by proteomic and metabolomic analysis: PROMIS. Under test this method was able to identify known PMIs such as enzyme–cofactor complexes as well as novel ones.
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Acknowledgments
We thank Hezi Tenenboim for his excellent help with English editing.
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Luzarowski, M., Sokolowska, E.M., Schlossarek, D., Skirycz, A. (2023). PROMIS: Co-fractionation Mass Spectrometry for Analysis of Protein–Metabolite Interactions. In: Skirycz, A., Luzarowski, M., Ewald, J.C. (eds) Cell-Wide Identification of Metabolite-Protein Interactions. Methods in Molecular Biology, vol 2554. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2624-5_10
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DOI: https://doi.org/10.1007/978-1-0716-2624-5_10
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