Abstract
We describe a simple stable isotope dilution method for accurate and precise measurement of cerebrospinal fluid (CSF) lactate as a clinical diagnostic test. Lactate is produced from cellular metabolism, primarily in muscle cells, and provides a source of energy especially during instances of low oxygen levels. Measurement of lactate in CSF provides diagnostic information regarding the body’s oxidative metabolism including diagnosis of lactate acidosis, aiding in the diagnosis of blood–brain barrier glucose transporter defect and differentiation between bacterial and viral meningitis. Determination of lactate in CSF (20 μL) was performed utilizing high-performance liquid chromatography coupled with electrospray positive ionization tandem mass spectrometry (HPLC–ESI–MS/MS). Lactate in CSF is determined by a 1:10 dilution with internal standard (sodium lacate-d3) and injected directly onto the HPLC–ESI–MS/MS system. Each assay is quantified using a six-point standard curve (0.625–20 mM) and has an analytical measurement range of 0.3–20 mM.
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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Wasek, B., Arning, E. (2022). Quantitation of Lactate in Cerebrospinal Fluid Using Liquid Chromatography–Electrospray-Tandem Mass Spectrometry. In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Biomolecular Analysis. Methods in Molecular Biology, vol 2546. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2565-1_23
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DOI: https://doi.org/10.1007/978-1-0716-2565-1_23
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2564-4
Online ISBN: 978-1-0716-2565-1
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