Abstract
Transmission electron microscopy (TEM) is the method of choice to image the ultrastructure of cells or tissues. TEM allows the visualization of molecular complexes up to an atomic resolution. Thus, TEM data have led to important conclusions about cellular processes and supported findings obtained by functional analyses. In this chapter, we describe the preparation of Drosophila tissues for TEM and provide reliable step-by-step protocols for applying classical chemical fixation or high-pressure freezing–freeze substitution (HPF–FS) to preserve cellular structures.
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Acknowledgments
We thank Kerstin Etzold, Birgit Hemmis, Mechthild Krabusch, Martina Biedermann, and Werner Mangerich for expert technical assistance. Furthermore, we thank Christian Meyer and Jonas Olbrich for sharing their TEM expertise with us. This work was supported by grants from the DFG (Deutsche Forschungsgemeinschaft) to A.P. (PA517/13-1, PA517/15-1, PA517/16-1, SFB 944-TP7, SFB 944 Z-Project). Our TEM protocols were initially compiled from Tepass and Hartenstein (1994); McDonald, Sharp, and Rickoll (2000); Lehmacher (2009, 2012), and many other authors.
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Psathaki, OE., Paululat, A. (2022). Preparation of Drosophila Tissues and Organs for Transmission Electron Microscopy. In: Dahmann, C. (eds) Drosophila. Methods in Molecular Biology, vol 2540. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2541-5_19
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DOI: https://doi.org/10.1007/978-1-0716-2541-5_19
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