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Quantitative Chemical Imaging at the Cellular Level: SIMS, Fluorescence, and Correlative Techniques

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Single Cell ‘Omics of Neuronal Cells

Part of the book series: Neuromethods ((NM,volume 184))

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Abstract

The cell is a heterogeneous chemical structure designed to accommodate its complex cellular functions in a living organism. Quantitative chemical imaging at the cellular level enables the investigation of the structural and functional molecular relation underlying cellular processes. We describe here the detailed methodology of the state-of-the-art secondary ion mass spectrometry (SIMS, NanoSIMS) and fluorescence microscopy (confocal, STED), along with selected examples for quantitative imaging at the cellular level. Correlative imaging that combines different imaging techniques is also demonstrated for selected applications in cell imaging. This chapter serves as a guideline assisting readers from unfamiliar fields of research to obtain reliable imaging at the cellular level while highlighting the strengths, limitations, and potentials of these technologies for cell imaging.

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Author information

Author notes

  1. Authors “Tho D.K. Nguyen” and “Alicia A. Lork” have equally contributed to this chapter.

Authors and Affiliations

  1. Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

    Tho D. K. Nguyen, Alicia A. Lork, Andrew G. Ewing & Nhu T. N. Phan

Authors
  1. Tho D. K. Nguyen
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  2. Alicia A. Lork
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  3. Andrew G. Ewing
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  4. Nhu T. N. Phan
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Corresponding authors

Correspondence to Andrew G. Ewing or Nhu T. N. Phan .

Editor information

Editors and Affiliations

  1. The Beckman Institute, University of Illinois Urbana-Champaign, Urbana, IL, USA

    Jonathan V. Sweedler

  2. Dept Pharmacology, 38 John Morgan Bldg, University of Pennsylvania, Philadelphia, PA, USA

    James Eberwine

  3. Bridge Institute, University of Southern California, Los Angeles, CA, USA

    Scott E. Fraser

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Nguyen, T.D.K., Lork, A.A., Ewing, A.G., Phan, N.T.N. (2022). Quantitative Chemical Imaging at the Cellular Level: SIMS, Fluorescence, and Correlative Techniques. In: Sweedler, J.V., Eberwine, J., Fraser, S.E. (eds) Single Cell ‘Omics of Neuronal Cells. Neuromethods, vol 184. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2525-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-2525-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2524-8

  • Online ISBN: 978-1-0716-2525-5

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