Abstract
Metagenomics offers the possibility to study the microbial community of soils at large scale, allowing the characterization also of unculturable microorganisms. Availability of high-quality DNA is a prerequisite of this approach. However, since soils have highly heterogeneous physicochemical properties, several parameters need to be considered for the development of the best molecular practices. A method for isolation of high-molecular-weight and good-quality metagenomic DNA from different soil samples is described here. The protocol combines physical and chemical strategies to ensure efficient cell lysis and precipitation of humic impurities-free DNA suitable for downstream processing for metagenomics study.
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Vettraino, A.M., Luchi, N. (2022). A Rapid Method for Extracting High-Quality DNA from Soils. In: Luchi, N. (eds) Plant Pathology. Methods in Molecular Biology, vol 2536. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2517-0_6
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DOI: https://doi.org/10.1007/978-1-0716-2517-0_6
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2516-3
Online ISBN: 978-1-0716-2517-0
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