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Somatic Embryogenesis as a Tool for Studying Grapevine–Virus Interaction

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Plant Pathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2536))

Abstract

More than 80 viral species, many of which are not associated with a clear disease or symptomatology, can infect grapevine. The study of grapevine–virus interactions in recent years is playing an increasingly important role and these studies have shown that the molecular and physiological responses to a virus greatly vary depending on the viral strains, the presence of multiple viral infections, the grapevine genotype, and the environment. Moreover, due to the characteristics of the grapevine cultivation and its vegetative propagation, it is very difficult to find healthy plants in vineyards to use them as control in the experiments. Starting from these considerations, in order to investigate the plant–virus interaction in an unbiased way, it is important to set up an experimental system able to control as much of these variables as possible. The protocol here proposed provides the overcome some of these factors by: (i) the production of healthy plants by somatic embryogenesis; (ii) the virus transmission using in vitro micrografting, and (iii) the transfer of in vitro plants to ex-vitro conditions for the analysis of interest.

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Authors and Affiliations

  1. National Research Council, Institute for Sustainable Plant Protection (CNR-IPSP), Torino, Italy

    Giorgio Gambino & Irene Perrone

Authors
  1. Giorgio Gambino
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  2. Irene Perrone
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Corresponding author

Correspondence to Giorgio Gambino .

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Editors and Affiliations

  1. Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Sesto Fiorentino, Florence, Italy

    Nicola Luchi

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Gambino, G., Perrone, I. (2022). Somatic Embryogenesis as a Tool for Studying Grapevine–Virus Interaction. In: Luchi, N. (eds) Plant Pathology. Methods in Molecular Biology, vol 2536. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2517-0_21

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  • DOI: https://doi.org/10.1007/978-1-0716-2517-0_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2516-3

  • Online ISBN: 978-1-0716-2517-0

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