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Analysis of ER-Phagy in Cancer Drug Resistance

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Cancer Drug Resistance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2535))

Abstract

The ability of the cancer cells to survive hostile environment depends on their cellular stress response mechanisms. These mechanisms also help them to develop resistance to chemotherapies. Autophagy and more specifically organelle specific autophagy is one such adaptive mechanism that promotes drug resistance in cancer cells. Endoplasmic reticulum–specific autophagy or ER-phagy has been more recently described to overcome ER-stress through the degradation of damaged ER. ER-resident proteins such as FAM134B act as ER-phagy receptors to specifically target damaged ER for degradation through autophagy. Moreover, we had recently deciphered that ER-phagy facilitates cancer cell survival during hypoxic stress and we predict that this process could play a critical role in the development of drug resistance in cancer cells. Therefore, here, we provide a lay description of how ER-phagy could be investigated biochemically by Western blot analysis and silencing ER-phagy receptor genes using small interfering RNAs (siRNA).

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Acknowledgments

Research in NR’s laboratory is supported by funds from Centre for Cancer Biology, University of South Australia, and the Neurosurgical Research Foundation (NRF), Australia. The authors also acknowledge the support to SC from Department of Science and Technology, Government of India (SR/WOS-A/LS-21/2016) under Women Scientist Scheme. The authors also acknowledge that the graphical representations were created with BioRender.com.

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Author notes

  1. Sandhya Chipurupalli

    Present address: Departments of Pediatrics & Biochemistry and Molecular Biology, Atlantic Research Centre, Dalhousie University, Halifax, NS, Canada

Authors and Affiliations

  1. Cellular Stress and Immune Response Laboratory, Centre for Cancer Biology, University of South Australia and SA Pathology, Adelaide, SA, Australia

    Sandhya Chipurupalli & Nirmal Robinson

  2. Department of Experimental Medicine, University of Campania “L. Vanvitelli”, Naples, Italy

    Vincenzo Desiderio

Authors
  1. Sandhya Chipurupalli
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  2. Vincenzo Desiderio
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  3. Nirmal Robinson
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Corresponding author

Correspondence to Nirmal Robinson .

Editor information

Editors and Affiliations

  1. Department of Oncology and Molecolar Medicine, Istituto Superiore di Sanità, Rome, Italy

    Marta Baiocchi

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Chipurupalli, S., Desiderio, V., Robinson, N. (2022). Analysis of ER-Phagy in Cancer Drug Resistance. In: Baiocchi, M. (eds) Cancer Drug Resistance. Methods in Molecular Biology, vol 2535. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2513-2_16

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  • DOI: https://doi.org/10.1007/978-1-0716-2513-2_16

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2512-5

  • Online ISBN: 978-1-0716-2513-2

  • eBook Packages: Springer Protocols

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