Abstract
We describe a protocol for H3K27ac ChIP paired-end sequencing and computational analysis of rearrangements. Our approach can be used to simultaneously map enhancers and their activity and to identify structural variations at enhancers. Since changes in enhancer activity and new enhancer translocations both play a major role in tumor initiation, progression, and response to therapy, this approach holds promise to uncover some of the mechanisms behind these processes.
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Acknowledgments
This chapter is based upon work support by a grant from the Neuroendocrine Tumor Research Foundation, and by grant #1049/20 from the Israel Science Foundation.
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Antman, I., Davis, E., Abu-Kamel, S., Hecht, M., Drier, Y. (2022). Simultaneous Mapping of Enhancers and Enhancer Rearrangements with Paired-End H3K27ac ChIP-seq. In: Baiocchi, M. (eds) Cancer Drug Resistance. Methods in Molecular Biology, vol 2535. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2513-2_11
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DOI: https://doi.org/10.1007/978-1-0716-2513-2_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2512-5
Online ISBN: 978-1-0716-2513-2
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