Abstract
A high-throughput quantitative determination of multiple light-emitting samples is a virtue of many light determination systems. In this chapter, we introduce a compact and efficient light determination system with a microsliding platform, a single-channel photomultiplier tube (PMT), and the controlling software for quantitative imaging of low-energy lights. The microsliding platform is uniquely designed to hold a multichannel microslide or an 8-lane PCR tube strip. The platform supports consecutive measurement of the multiple light samples through sliding the microslide or the PCR tube strip like a conveyor belt. We exemplify determination of multiple alkaline phosphatase samples and single-chain bioluminescent probes using this system. We also outline the mechanical specification of the system. This unique luminometer is an important addition to compact on-site quantitative light determination systems that are useful in various research fields including analytical chemistry, biology, and basic science in medicine.
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Acknowledgments
This work was supported in part by JSPS KAKENHI Grants: Numbers 15KK0029, 17H01215, 20Â K21851, and 21H04948. We thank Dr. Simon Miller, Dr. Toshiya Senda, and Dr. Mikio Tanabe (Structural Biology Research Center, Institute of Materials Structure Science, High-Energy Accelerator Research Organization (KEK)) for preparation of purified FRB-A23-FKBP sample. This work was also partly supported by Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS) from the Japan Agency for Medical Research and Development (AMED) under grant numbers JP21am0101071.
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Kim, SB., Paulmurugan, R. (2022). One-Channel Microsliding Luminometer for Quantifying Low-Energy Bioluminescent Lights. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2525. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2473-9_28
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DOI: https://doi.org/10.1007/978-1-0716-2473-9_28
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2472-2
Online ISBN: 978-1-0716-2473-9
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