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Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2524))

Abstract

The present protocol introduces a visible light bioluminescence imaging (BLI) platform together with 12 novel coelenterazine (CTZ) analogues and luciferase sets. We exemplify to create diverse hues of bioluminescence (BL) ranging from blue to far red with the combination of marine luciferases and the three groups of CTZ analogues. We also show how to characterize the new CTZ analogues in detail such as the kinetic parameters, dose dependency, and luciferase specificity. The 2-series CTZ analogues interestingly have specificity to artificial luciferases and are completely dark with Renilla luciferase derivatives in contrast. The 3d is highly specific to only NanoLuc. This BL imaging system covering the visible region provides a useful multicolor imaging portfolio that efficiently images molecular events in mammalian cells.

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Acknowledgments

This work was supported in part by JSPS KAKENHI Grants: Numbers 15KK0029, 17H01215, 20K21851, and 21H04948.

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Authors and Affiliations

  1. Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu, Tokyo, Japan

    Nobuo Kitada & Shojiro Maki

  2. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

Authors
  1. Nobuo Kitada
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  2. Shojiro Maki
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  3. Sung-Bae Kim
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Corresponding author

Correspondence to Sung-Bae Kim .

Editor information

Editors and Affiliations

  1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Kitada, N., Maki, S., Kim, SB. (2022). Visible Light Bioluminescence Imaging Platform for Animal Cell Imaging. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2524. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2453-1_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2453-1_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2452-4

  • Online ISBN: 978-1-0716-2453-1

  • eBook Packages: Springer Protocols

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