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Imaging of Autonomous Bioluminescence Emission From Single Mammalian Cells

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2524))

Abstract

The bioluminescent visualization of individual mammalian cells usually requires the addition of a luciferin substrate. This chapter describes the microscopic imaging of single cells by their bioluminescence (BL) emission generated without an external luciferin. Imaging is based on the expression of codon-optimized lux (co lux) genes and does not require manipulation of the cells apart from transfection. Due to the high brightness of the co lux system, light emission from single cells can be observed continuously for many hours using a specialized microscope.

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Acknowledgments

I thank Prof. Stefan W. Hell for the opportunity to carry out this work in his department at the Max Planck Institute for Biophysical Chemistry in Göttingen. This work was funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy - EXC 2067/1- 390729940.

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Authors and Affiliations

  1. Department of Optical Nanoscopy, Institut für Nanophotonik Göttingen e.V, Göttingen, Germany

    Carola Gregor

  2. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

    Carola Gregor

  3. Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC), University of Göttingen, Göttingen, Germany

    Carola Gregor

Authors
  1. Carola Gregor
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Corresponding author

Correspondence to Carola Gregor .

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Editors and Affiliations

  1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

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Gregor, C. (2022). Imaging of Autonomous Bioluminescence Emission From Single Mammalian Cells. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2524. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2453-1_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2453-1_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2452-4

  • Online ISBN: 978-1-0716-2453-1

  • eBook Packages: Springer Protocols

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