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Harnessing CRISPR-Cas9 for Epigenetic Engineering

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Riboregulator Design and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2518))

Abstract

Epigenome editing has become more precise and effective by coupling epigenetic effectors to the dCas9 protein and targeting regulatory regions such as promoters and enhancers. Here, we describe a basic methodology for performing an epigenome editing experiment, starting from gRNA design and cloning to transiently transfecting the gRNA plasmid and the CRISPR/dCas9-based epigenetic effector and finalizing with chromatin immunoprecipitation (ChIP) to validate changes in epigenetic state at a targeted genomic region.

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Author information

Authors and Affiliations

  1. Systems, Synthetic, and Physical Biology PhD Program, Rice University, Houston, TX, USA

    Rosa S. Guerra-Resendez & Isaac B. Hilton

  2. Department of Bioengineering, Rice University, Houston, TX, USA

    Isaac B. Hilton

  3. Department of BioSciences, Rice University, Houston, TX, USA

    Isaac B. Hilton

Authors
  1. Rosa S. Guerra-Resendez
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  2. Isaac B. Hilton
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Corresponding author

Correspondence to Isaac B. Hilton .

Editor information

Editors and Affiliations

  1. Biosciences Department, Rice University, Houston, TX, USA

    James Chappell

  2. Department of Biology, California State University, Northridge, Northridge, CA, USA

    Melissa K. Takahashi

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Guerra-Resendez, R.S., Hilton, I.B. (2022). Harnessing CRISPR-Cas9 for Epigenetic Engineering. In: Chappell, J., Takahashi, M.K. (eds) Riboregulator Design and Analysis. Methods in Molecular Biology, vol 2518. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2421-0_14

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  • DOI: https://doi.org/10.1007/978-1-0716-2421-0_14

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2420-3

  • Online ISBN: 978-1-0716-2421-0

  • eBook Packages: Springer Protocols

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