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Susceptibility Testing of Candida auris Isolates

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Candida auris

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2517))

Abstract

Susceptibility testing of isolates of Candida auris is helpful as a guide to the selection of the most appropriate antifungal agent for treatment as different clades and strains within clades often demonstrate markedly different susceptibility profiles. Some strains are relatively susceptible to all antifungal drugs, but most demonstrate innate resistance to fluconazole, many are cross-resistant to other azoles and others demonstrate resistance to other classes of antifungal. The finding of multi-drug resistance, where an isolate is resistant to two or more classes of antifungal agent, is not uncommon, and development of resistance during a course of treatment has also been documented. This chapter describes a reference broth microdilution method for susceptibility testing and a commercially available gradient strip method.

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Acknowledgments

We thank the staff of the UK National Mycology Reference Laboratory for their expertise in conducting these methods, for providing much of the data helping to validate the methods for this species and for preparing detailed SOPs. Thank you also to Associate Professor Annette Fothergill from The Fungus Testing laboratory, San Antonio, Texas, USA, who participated in the introduction of the CLSI plate dilution preparation method into our laboratory.

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Authors and Affiliations

  1. UK National Mycology Reference Laboratory, Public Health England South-West Regional Laboratory, Southmead Hospital, Bristol, UK

    Elizabeth M. Johnson & Andrew M. Borman

  2. MRC Centre for Medical Mycology, University of Exeter, Exeter, UK

    Elizabeth M. Johnson & Andrew M. Borman

Authors
  1. Elizabeth M. Johnson
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  2. Andrew M. Borman
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Corresponding author

Correspondence to Elizabeth M. Johnson .

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Editors and Affiliations

  1. Institute of Medical Sciences (IMS), University of Aberdeen, Aberdeen, UK

    Alexander Lorenz

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Johnson, E.M., Borman, A.M. (2022). Susceptibility Testing of Candida auris Isolates. In: Lorenz, A. (eds) Candida auris. Methods in Molecular Biology, vol 2517. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2417-3_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2417-3_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2416-6

  • Online ISBN: 978-1-0716-2417-3

  • eBook Packages: Springer Protocols

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