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Modeling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel–Collagen I Cultures

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Cancer Cell Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2508))

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Abstract

Understanding the modes and mechanisms of tumor cell invasion is key to developing targeted therapies against metastatic disease. In vitro assays modeling tumor progression have primarily been optimized for studying classical single-cell migration through an epithelial–mesenchymal transition (EMT). Although experimental and clinical histopathological evidence has revealed that tumor invasion is plastic and that epithelial carcinomas can invade by a range of modes that vary from single, mesenchyme-like cells, all the way to cohesive, collective units, few in vitro assays have been designed to assess these modes specifically. Thus, we have developed a Matrigel–Collagen I overlay assay that is suitable for identifying and quantifying both collective and mesenchymal invasion. This three-dimensional (3D) culture assay utilizes the features of Matrigel and Collagen I to mimic the laminin-rich basement membrane and the stiff, fibrillar Collagen I tumor microenvironment allowing for spheroid invasion to be assessed at the interface between these two matrix components.

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Acknowledgments

This work was supported by grants from the Canadian Institutes of Health Research (CIHR), Canadian Cancer Society-Research (CCS-R), and the Cancer Research Society (CRS). E.M.B. was supported by a doctoral scholarship from the Natural Sciences and Engineering Research Council of Canada (CGS-D NSERC).

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Authors and Affiliations

  1. Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC, Canada

    Erin M. Bell, Pamela M. Dean, Tate O. Goodman & Calvin D. Roskelley

  2. Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada

    Marcia L. Graves

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  1. Erin M. Bell
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  2. Marcia L. Graves
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  3. Pamela M. Dean
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  4. Tate O. Goodman
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  5. Calvin D. Roskelley
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Correspondence to Calvin D. Roskelley .

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Editors and Affiliations

  1. Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada

    Sherri L. Christian

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Bell, E.M., Graves, M.L., Dean, P.M., Goodman, T.O., Roskelley, C.D. (2022). Modeling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel–Collagen I Cultures. In: Christian, S.L. (eds) Cancer Cell Biology. Methods in Molecular Biology, vol 2508. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2376-3_8

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  • DOI: https://doi.org/10.1007/978-1-0716-2376-3_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2375-6

  • Online ISBN: 978-1-0716-2376-3

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