Abstract
Lung slices have been used since the mid-1990’s to study various aspects of lung biology that include, but are not limited to, mechanisms of airway contraction and relaxation; the pulmonary immune response in the context of inflammatory diseases of the lung like asthma and chronic obstructive pulmonary disease; mast cell-mediated airway contractility and inflammation; modulation of airway cells following pathogen exposure; and consequences of environmental toxicant exposure. Here we describe the generation of human precision-cut lung slices (hPCLS) and measurement of contraction and relaxation of small airways within the slices.
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Acknowledgments
This work was supported by NIH P01-HL114471, UL-TR003017, and R56-HL142890.
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1 Electronic Supplementary Material
Video showing contracting airways https://www.dropbox.com/s/vlt6zweih9v7lgf/ige%20crosslinking%20images.mp4?dl=0 (MP4 3523 kb)
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Koziol-White, C. (2022). Human Precision-Cut Lung Slices: Generation of and Measurement of Contractility and Relaxation of Small Airways. In: Gorska, M.M. (eds) Asthma. Methods in Molecular Biology, vol 2506. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2364-0_8
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DOI: https://doi.org/10.1007/978-1-0716-2364-0_8
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2364-0
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