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A Novel Nonhuman Primate Model of Nonatopic Asthma

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Asthma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2506))

Abstract

Nonhuman primate models have an essential role in understanding progressive respiratory disease pathogenesis. Immune and physiologic parameters in the nonhuman primate closely reflect the complexity of human systems and provide an exceptional translational impact for the investigation of the mucosal immune changes in response to environmental exposures. This potential warrants the development of novel models that will clarify the interaction of respiratory disease and the inhalable environment and the potential of novel therapies to alleviate the untoward results of these interactions. Nonhuman primate models of asthma can be spontaneous, induced, or experimentally manipulated by various exposures. Here we describe a model of exacerbation of airway hyperreactivity induced by exposure to an air pollutant, ozone, in a cohort of young adult asthmatic rhesus macaques.

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Abbreviations

AHR:

Airway hyperreactivity

BAL:

Bronchoalveolar lavage

CNPRC:

California National Primate Research Center

DC:

Dendritic cell

FACS:

Fluorescent activated cell sorting

IL-:

interleukin-

ILC:

Innate lymphoid cell

mDC:

myeloid DC

Nrf2:

nuclear factor-like 2

O3:

Ozone

pDC:

plasmacytoid DC

ppm:

parts per million

qPCR:

Quantitative real-time polymerase chain reaction

Raw:

airway resistance to methacholine

SP-D:

Surfactant protein-D

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Acknowledgement

R21AI116121 and 1R41AI132012-01 (Angela Haczku), CNPRC base operating grant P51OD011107 (Prasant Mohapatra), and K01OD024782 (Christopher Royer).

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Authors and Affiliations

  1. University of California, Davis, Davis, CA, USA

    Christopher Royer, Lisa A. Miller & Angela Haczku

Authors
  1. Christopher Royer
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  2. Lisa A. Miller
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  3. Angela Haczku
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Corresponding author

Correspondence to Angela Haczku .

Editor information

Editors and Affiliations

  1. Division of Allergy and Clinical Immunology, Department of Medicine, National Jewish Health, Denver, CO, USA

    Magdalena M. Gorska

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Royer, C., Miller, L.A., Haczku, A. (2022). A Novel Nonhuman Primate Model of Nonatopic Asthma. In: Gorska, M.M. (eds) Asthma. Methods in Molecular Biology, vol 2506. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2364-0_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2364-0_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2363-3

  • Online ISBN: 978-1-0716-2364-0

  • eBook Packages: Springer Protocols

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