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Quantification of Short-Chain Fatty Acids in Feces

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Basic Protocols in Foods and Nutrition

Part of the book series: Methods and Protocols in Food Science ((MPFS))

Abstract

Short-chain fatty acids (SCFA) have attracted the attention of researchers due to their positive physiological effects on health. SCFA are involved directly or indirectly in many physiologic responses correlated to the modulation of inflammatory processes, anti-carcinogenic effects, and cardiovascular disease risk-reducing. Besides that, SCFA have a regulatory role in the gut environment and hepatic and whole-body glucose homeostasis. Chemically, SCFA are carboxylic acids with an aliphatic chain containing two to six carbons. They are the primary products of gut fermentation of dietary fiber. Therefore, a quantitative analysis of SCFA in feces is necessary to evaluate the impact of fiber-rich foods on health. This chapter aims to provide some of the principal basic protocols for extracting, separating, and quantifying SCFA in feces.

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Acknowledgements

The authors would like to thank the São Paulo Research Foundation (FAPESP—Ph.D. grant for M.R.V.B., 2019/18748-8) and to Coordination for the Improvement of Higher Education Personnel (CAPES Foundation—Brazil) Financing Code 001.

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Authors and Affiliations

  1. University of São Paulo (USP), São Carlos Institute of Chemistry (IQSC), São Carlos, São Paulo, Brazil

    Mirella Romanelli Vicente Bertolo & Stanislau Bogusz Junior

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  1. Mirella Romanelli Vicente Bertolo
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  2. Stanislau Bogusz Junior
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Correspondence to Stanislau Bogusz Junior .

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Editors and Affiliations

  1. Faculty of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil

    Cinthia Baú Betim Cazarin

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Bertolo, M.R.V., Bogusz Junior, S. (2022). Quantification of Short-Chain Fatty Acids in Feces. In: Betim Cazarin, C.B. (eds) Basic Protocols in Foods and Nutrition. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2345-9_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2345-9_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2344-2

  • Online ISBN: 978-1-0716-2345-9

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