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The GSH Colorimetric Method as Measurement of Antioxidant Status in Serum and Rodent Tissues

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Basic Protocols in Foods and Nutrition

Part of the book series: Methods and Protocols in Food Science ((MPFS))

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Abstract

Aerobic organisms have a wide variety of antioxidants in the biological systems to protect themselves against oxidative stress, GSH being one of the most important component. In this method, GSH reacts with the Ellman’s reagent producing 2-nitro-5-mercapto-benzoic acid (TNB), which possesses its absorption maximum at 412 nm, showing a yellow-colored reaction. This assay allows colorimetric detection of almost entire thiol groups in several tissues. This chapter describes optimized conditions for testing rodent tissue homogenates and serum using Ellman’s reagent-based GSH method. The Ellman’s GSH method is a rapid, inexpensive, and accurate method to measure in vivo antioxidant status.

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Authors and Affiliations

  1. Department of Food Science and Nutrition, School of Food Engineering, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Milena Morandi Vuolo

  2. Department of Nutrition, Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN, Brazil

    Juliana Kelly da Silva-Maia

  3. Department of Food and Nutrition, Universidade Federal de Santa Maria (UFSM), Palmeira das Missões, RS, Brazil

    Ângela Giovana Batista

Authors
  1. Milena Morandi Vuolo
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  2. Juliana Kelly da Silva-Maia
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  3. Ângela Giovana Batista
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Corresponding author

Correspondence to Ângela Giovana Batista .

Editor information

Editors and Affiliations

  1. Faculty of Food Engineering, University of Campinas, Campinas, São Paulo, Brazil

    Cinthia Baú Betim Cazarin

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Vuolo, M.M., da Silva-Maia, J.K., Batista, Â.G. (2022). The GSH Colorimetric Method as Measurement of Antioxidant Status in Serum and Rodent Tissues. In: Betim Cazarin, C.B. (eds) Basic Protocols in Foods and Nutrition. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2345-9_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2345-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2344-2

  • Online ISBN: 978-1-0716-2345-9

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