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Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins

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Rhodopsin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2501))

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Abstract

Optogenetics allows control of neural activity in genetically targeted neuron populations by light. Optogenetic control of individual neurons in neural circuits would enable powerful, causal investigations of neural connectivity and function at single-cell level and provide insights into how neural circuits operate. Such single-cell resolution optogenetics in neuron populations requires precise sculpting of light and subcellular targeting of optogenetic molecules. Here we describe a group of methods for single-cell resolution optogenetics in neuron cultures, in mouse brain slices, and in mouse cortex in-vivo, via patterned light and soma-targeted optogenetic molecules.

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Acknowledgments

We thank the IHU FOReSIGHT grant (Grant P-ALLOP3-IHU-000), the National Institute of Health (Grant NIH 1UF1NS107574 - 01), the “Agence National de la Recherche” ANR (project PRCI), and the Axa research funding.

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Authors and Affiliations

  1. McGovern Institute for Brain Research, MIT, Cambridge, MA, USA

    Changyang Linghu & Or A. Shemesh

  2. Sorbonne Université, INSERM, CNRS, Institut de la Vision, Paris, France

    I-Wen Chen, Dimitrii Tanese & Valeria Zampini

  3. Department of Neurobiology, Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    Or A. Shemesh

Authors
  1. Changyang Linghu
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  2. I-Wen Chen
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  3. Dimitrii Tanese
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  4. Valeria Zampini
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  5. Or A. Shemesh
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Corresponding author

Correspondence to Or A. Shemesh .

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Editors and Affiliations

  1. Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France

    Valentin Gordeliy

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Linghu, C., Chen, IW., Tanese, D., Zampini, V., Shemesh, O.A. (2022). Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins. In: Gordeliy, V. (eds) Rhodopsin. Methods in Molecular Biology, vol 2501. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2329-9_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2329-9_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2328-2

  • Online ISBN: 978-1-0716-2329-9

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