Abstract
Mitochondrial calcium (Ca2+) plays a key role in regulating normal cardiac function. A physiological increase in mitochondrial matrix calcium [Ca2+]m drives mitochondrial ATP production to meet the high-energy demands during excitation–contraction coupling. However, a pathological increase in [Ca2+]m leads to increased oxidative stress, impaired bioenergetics, and the opening of mitochondrial permeability transition pore (mPTP), a hallmark of the failing heart. Therefore, a better understanding of the [Ca2+]m handling and its role in heart function and dysfunction is of great importance. Here, we describe a detailed protocol for measuring mitochondrial Ca2+ handling in the isolated functionally intact mitochondria from cardiac tissue of the guinea pig.
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Acknowledgments
We would like to acknowledge James Heisner for setting up and optimizing the approaches described here. This work was supported by a grant from NIH: R01 HL131673 to AKSC.
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Mishra, J., Camara, A.K.S. (2022). Mitochondrial Calcium Handling in Isolated Mitochondria from a Guinea Pig Heart. In: Tomar, N. (eds) Mitochondria. Methods in Molecular Biology, vol 2497. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2309-1_6
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DOI: https://doi.org/10.1007/978-1-0716-2309-1_6
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Publisher Name: Humana, New York, NY
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