Abstract
The mitochondrial membrane potential (ΔΨm) generated by proton pumps (Complexes I, III, and IV) is an essential component in the process of energy generation during oxidative phosphorylation. Tetramethylrhodamine, methyl ester, perchlorate (TMRM) is one of the most commonly used fluorescent reporters of ΔΨm. TMRM is routinely employed in a steady state for the measurement of membrane potential. However, it can also be utilized with time-lapse fluorescence imaging to effectively monitor the changes in membrane potential in response to a given stimulus by analyzing the change in distribution of the dye with time.
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Acknowledgments
Funding for assay development and manuscript preparation was provided by a scholarship from the National Agency for Research and Development (ANID)/Scholarship Program/DOCTORADO BECAS CHILE/2019—7220052 which supports GEV.
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Valdebenito, G.E., Duchen, M.R. (2022). Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging. In: Tomar, N. (eds) Mitochondria. Methods in Molecular Biology, vol 2497. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2309-1_22
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DOI: https://doi.org/10.1007/978-1-0716-2309-1_22
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