Abstract
Abiotic stress impacts a wide range of plant developmental processes. Among them, cell expansion is particularly important given its contribution to plant growth and morphogenesis. Here, we describe a new phenotypic system to quantify accurately the impact of different sources of abiotic stress on the cell’s capacity to expand. This approach monitors hypocotyl growth in Arabidopsis thaliana etiolated seedlings, as in the dark this embryonic organ is known to grow solely by expanding its cells, without cell division.
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Acknowledgments
G.M. was supported by an EMBO Long-Term Fellowship (ALTF 1576-2016) and a Marie Skłodowska-Curie Individual Postdoctoral Fellowship (EU project 750469). Work in our lab is funded by Fundação para a Ciência e a Tecnologia (FCT) through grants PTDC/BIA-FBT/31018/2017 and PTDC/BIA-BID/30608/2017. Funding from the research unit GREEN-it “Bioresources for Sustainability” (UIDB/04551/2020) is also acknowledged.
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Martín, G., Duque, P. (2022). Etiolated Hypocotyls: A New System to Study the Impact of Abiotic Stress on Cell Expansion. In: Duque, P., Szakonyi, D. (eds) Environmental Responses in Plants. Methods in Molecular Biology, vol 2494. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2297-1_13
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DOI: https://doi.org/10.1007/978-1-0716-2297-1_13
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