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An hiPSC-Derived In Vitro Model of the Blood–Brain Barrier

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The Blood-Brain Barrier

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2492))

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Abstract

Human induced pluripotent stem cells (hiPSC) offer a tractable system to model the blood–brain barrier (BBB). Here we detail the assembly of a triple co-culture hiPSC-BBB model, using hiPSC-derived brain microvascular endothelial cells (BMEC), astrocytes, and mural cells (MC). Transendothelial electrical resistance (TEER) and sodium fluorescein (NaFl) permeability can be used to test the barrier properties. The model has applications in studying BBB-related pathology and for drug screening.

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Acknowledgements

This work was supported by the Rosetrees Trust, British Heart Foundation, Alzheimer’s Association and Stroke Association.

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Authors and Affiliations

  1. Stroke Research Group, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK

    Mary Goodwin-Trotman, Krushangi Patel & Alessandra Granata

Authors
  1. Mary Goodwin-Trotman
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  2. Krushangi Patel
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  3. Alessandra Granata
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Corresponding author

Correspondence to Mary Goodwin-Trotman .

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Editors and Affiliations

  1. Discovery Pharmacology, COMPASS Pathways PLC, London, UK

    Nicole Stone

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Goodwin-Trotman, M., Patel, K., Granata, A. (2022). An hiPSC-Derived In Vitro Model of the Blood–Brain Barrier. In: Stone, N. (eds) The Blood-Brain Barrier. Methods in Molecular Biology, vol 2492. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2289-6_5

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  • DOI: https://doi.org/10.1007/978-1-0716-2289-6_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2288-9

  • Online ISBN: 978-1-0716-2289-6

  • eBook Packages: Springer Protocols

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