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Yeast Surface Display

  • Book
  • © 2022

Overview

Editors:
  1. Michael W. Traxlmayr

    1. Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, Vienna, Austria

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2491)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xv
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  2. Introduction to Yeast Surface Display and Its Applications

    1. Front Matter

      Pages 1-1
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    2. Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System

      • Maryam Raeeszadeh-Sarmazdeh, Eric T. Boder
      Pages 3-25

  3. Construction and Selection of Yeast Surface Display Libraries and Analysis of Isolated Variants

    1. Front Matter

      Pages 27-27
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    2. Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation

      • Byong H. Kang, Brianna M. Lax, K. Dane Wittrup
      Pages 29-62

    3. Site-wise Diversification of Combinatorial Libraries Using Insights from Structure-guided Stability Calculations

      • Benedikt Dolgikh, Daniel Woldring
      Pages 63-73

    4. Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution

      • James VanAntwerp, Patrick Finneran, Benedikt Dolgikh, Daniel Woldring
      Pages 75-86

    5. Machine Learning-driven Protein Library Design: A Path Toward Smarter Libraries

      • Mehrsa Mardikoraem, Daniel Woldring
      Pages 87-104

    6. Kinetic Competition Screening of Yeast-Displayed Libraries for Isolating High Affinity Binders

      • Nicole J. Yang
      Pages 105-115

    7. Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display

      • Preeti Sharma, Erik Procko, David M. Kranz
      Pages 117-142

    8. Engineering Binders with Exceptional Selectivity

      • Kai Wen Teng, Akiko Koide, Shohei Koide
      Pages 143-154

    9. Affinity and Stability Analysis of Yeast Displayed Proteins

      • Charlotte U. Zajc, Magdalena Teufl, Michael W. Traxlmayr
      Pages 155-173

  4. Selection of Yeast Surface Display Libraries for Binding to Mammalian Cells or Extracellular Matrix

    1. Front Matter

      Pages 175-175
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    2. Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting

      • Stefania C. Carrara, Jan P. Bogen, Julius Grzeschik, Björn Hock, Harald Kolmar
      Pages 177-193

    3. A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins

      • Patrick J. Krohl, Jamie B. Spangler
      Pages 195-216

    4. Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques

      • Rojhae A. Panton, Lawrence A. Stern
      Pages 217-233

    5. Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display

      • Benjamin J. Umlauf, John S. Kuo, Eric V. Shusta
      Pages 235-248

  5. Specialized Yeast Surface Display Applications

    1. Front Matter

      Pages 249-249
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    2. Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology

      • Sara Linciano, Ee Lin Wong, Ylenia Mazzocato, Monica Chinellato, Tiziano Scaravetti, Alberto Caregnato et al.
      Pages 251-262

    3. Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors

      • Brooke D. Huisman, Beth E. Grace, Patrick V. Holec, Michael E. Birnbaum
      Pages 263-291
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Keywords

About this book

This detailed volume explores a wide variety of applications of yeast surface display, an extensively used protein engineering technology. Beginning with detailed protocols for the construction and efficient selection/screening of yeast surface display libraries, as well as for the analysis of individual yeast-displayed protein variants, the book continues with protocols describing the selection of yeast surface display libraries for binding to mammalian cells or to extracellular matrix as well as protocols for a broad spectrum of specialized yeast surface display applications, demonstrating the versatility of this display platform. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible methodologies, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.

Editors and Affiliations

  • Department of Chemistry, Institute of Biochemistry, University of Natural Resources and Life Sciences, Vienna, Austria

    Michael W. Traxlmayr

Bibliographic Information

  • Book Title: Yeast Surface Display

  • Editors: Michael W. Traxlmayr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2285-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2284-1Published: 29 April 2022

  • Softcover ISBN: 978-1-0716-2287-2Published: 29 April 2023

  • eBook ISBN: 978-1-0716-2285-8Published: 28 April 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 646

  • Number of Illustrations: 13 b/w illustrations, 96 illustrations in colour

  • Topics: Protein Science

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