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Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host

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Engineering Natural Product Biosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2489))

Abstract

Plant natural products (PNPs) are valuable resources for the development of pharmaceuticals and agrochemicals, yet the biosynthesis and metabolism of PNPs are largely unknown. Heterologous pathway reconstitution is a heavily adopted strategy in secondary metabolism characterization. Yeast systems have been broadly utilized in the heterologous production of PNPs and have been considered as a promising platform to investigate plant biosynthetic pathways. Here, we describe the reconstitution and verification of the upstream part of brassinolide biosynthesis in S. cerevisiae using this method.

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Acknowledgments

We thank Dr. Christina Smolke from Stanford University for kindly providing pCS1056, pCS2812, pCS2814 and pCS3129 as gifts. We thank Anqi Zhou and Tiffany Chiu for valuable feedback in the preparation of the manuscript. This work was supported by Cancer Research Coordinating Committee Research Award (grant to Y.L., CRN-20-634571) and NIH New Innovator Award (grant to Y.L., DP2 AT011445-01).

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Author notes

  1. Shanhui Xu and Sheng Wu contributed equally to this chapter.

Authors and Affiliations

  1. Department of Chemical and Environmental Engineering, University of California, Riverside, CA, USA

    Shanhui Xu, Sheng Wu & Yanran Li

Authors
  1. Shanhui Xu
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  2. Sheng Wu
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  3. Yanran Li
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Corresponding author

Correspondence to Yanran Li .

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Editors and Affiliations

  1. Department of Chemistry & BioDiscovery Institute, University of North Texas, Denton, USA

    Elizabeth Skellam

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Xu, S., Wu, S., Li, Y. (2022). Investigating Plant Biosynthetic Pathways Using Heterologous Gene Expression: Yeast as a Heterologous Host. In: Skellam, E. (eds) Engineering Natural Product Biosynthesis. Methods in Molecular Biology, vol 2489. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2273-5_19

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  • DOI: https://doi.org/10.1007/978-1-0716-2273-5_19

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2272-8

  • Online ISBN: 978-1-0716-2273-5

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