Abstract
The methods of compartmentalized immobilization in multi-enzyme systems containing inorganic complexes and organic scaffolds (i.e. nucleic acid (RNA and DNA), protein and lipid) have been thoroughly investigated. Compartmentalization mostly focuses on dividing individual enzyme(s) into specific location or orientation of the enzymes cooperating in cascade reaction. Organic scaffolds are preferred because of their capability for simultaneous synthesis in biological systems. Besides, the most required methods of horseradish peroxidase (HRP) and glucose oxidase (GOD) enzymes including enzyme activity measurement, enzyme immobilization, removal, and re-hybridization, and enzyme attaching have been provided because they have been extensively applied in multi-enzyme systems. Organic scaffolds have a wide range and properties. Therefore, two methods including dockerin–cohesin linker and nucleotides interaction have been demonstrated for immobilization of enzyme on protein and DNA scaffold, respectively.
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References
Marguet M, Bonduelle C, Lecommandoux S (2013) Multicompartmentalized polymeric systems: towards biomimetic cellular structure and function. Chem Soc Rev 42:512–529
Wang L-B, Wang Y-C, He R et al (2013) A new nanobiocatalytic system based on allosteric effect with dramatically enhanced enzymatic performance. J Am Chem Soc 135:1272–1275
Ge J, Lei J, Zare RN (2012) Protein–inorganic hybrid nanoflowers. Nat Nanotechnol 7:428–432
Li Z, Zhang Y, Su Y et al (2014) Spatial co-localization of multi-enzymes by inorganic nanocrystal–protein complexes. Chem Commun 50:12465–12468
Schmitt C, Lippert AH, Bonakdar N et al (2016) Compartmentalization and transport in synthetic vesicles. Front Bioeng Biotechnol 4:19
Liu Z, Wang B, Jin S et al (2018) Bioinspired dual-enzyme colloidosome reactors for high-performance biphasic catalysis. ACS Appl Mater Interfaces 10:41504–41511
Mason AF, Yewdall NA, Welzen PLW et al (2019) Mimicking cellular compartmentalization in a hierarchical protocell through spontaneous spatial organization. ACS Cent Sci 5:1360–1365
Schmid-Dannert C, López-Gallego F (2019) Advances and opportunities for the design of self-sufficient and spatially organized cell-free biocatalytic systems. Curr Opin Chem Biol 49:97–104
Chen AH, Silver PA (2012) Designing biological compartmentalization. Trends Cell Biol 22:662–670
Li M, Yue Y, Zhang Z-J et al (2016) Site-specific and high-loading immobilization of proteins by using cohesin–dockerin and CBM–cellulose interactions. Bioconjug Chem 27:1579–1583
You C, Myung S, Zhang Y-HP (2012) Facilitated substrate channeling in a self-assembled trifunctional enzyme complex. Angew Chem Int Ed 51:8787–8790
Wilner OI, Weizmann Y, Gill R et al (2009) Enzyme cascades activated on topologically programmed DNA scaffolds. Nat Nanotechnol 4:249–254
Wang Y, Kim E, Lin Y et al (2019) Rolling circle transcription-amplified hierarchically structured organic–inorganic hybrid RNA flowers for enzyme immobilization. ACS Appl Mater Interfaces 11:22932–22940
Conrado RJ, Wu GC, Boock JT et al (2012) DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency. Nucleic Acids Res 40:1879–1889
Arnal, G., Attia, M. A., Asohan, J., & Brumer, H. (2017). A low-volume, parallel copper-bicinchoninic acid (BCA) assay for glycoside hydrolases. In Protein-Carbohydrate Interactions (pp. 3–14). Humana Press, New York, NY
Zhang Y, Li S-Z, Li J et al (2006) Using unnatural protein fusions to engineer resveratrol biosynthesis in yeast and mammalian cells. J Am Chem Soc 128:13030–13031
Maeder ML, Thibodeau-Beganny S, Osiak A et al (2008) Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell 31:294–301
Shetty RP, Endy D, Knight TF (2008) Engineering BioBrick vectors from BioBrick parts. J Biol Eng 2:5
Altaras NE, Cameron DC (1999) Metabolic engineering of a 1,2-propanediol pathway in Escherichia coli. Appl Environ Microbiol 65:1180–1185
Dueber JE, Wu GC, Malmirchegini GR et al (2009) Synthetic protein scaffolds provide modular control over metabolic flux. Nat Biotechnol 27:753–759
Beekwilder J, Wolswinkel R, Jonker H et al (2006) Production of resveratrol in recombinant microorganisms. Appl Environ Microbiol 72:5670–5672
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Baharifar, H., Khoshnevisan, K., Maleki, H. (2022). Compartmentalized Immobilization of Multi-enzyme Systems. In: Stamatis, H. (eds) Multienzymatic Assemblies. Methods in Molecular Biology, vol 2487. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2269-8_9
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DOI: https://doi.org/10.1007/978-1-0716-2269-8_9
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