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Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades

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Multienzymatic Assemblies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2487))

Abstract

Substrate or metabolite channelling is a transfer of intermediates produced by one enzyme to the sequential enzyme of a reaction cascade or metabolic pathway, without releasing them entirely into bulk. Despite an enormous effort and more than three decades of research, substrate channelling remains the subject of continuing debates and active investigation. Herein, we review the benefits and mechanisms of substrate channelling in vivo and in vitro. We discuss critically the effects that substrate channelling can have on enzymatic cascades, including speeding up or slowing down reaction cascades and protecting intermediates from sequestration and enzymes’ surroundings from toxic or otherwise detrimental intermediates. We also discuss how macromolecular crowding affects substrate channelling and point out the galore of open questions.

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Acknowledgements

This work was supported by National Science Centre grant No. 2017/25/B/ST3/02456 to S.K.

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Authors and Affiliations

  1. Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland

    Svyatoslav Kondrat

  2. Max-Planck-Institut für Intelligente Systeme, Stuttgart, Germany

    Svyatoslav Kondrat

  3. IV. Institut für Theoretische Physik, Universität Stuttgart, Stuttgart, Germany

    Svyatoslav Kondrat

  4. Forschungszentrum Jülich, IBG-1: Biotechnology, Jülich, Germany

    Eric von Lieres

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  1. Svyatoslav Kondrat
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  2. Eric von Lieres
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Correspondence to Svyatoslav Kondrat .

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  1. Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece

    Haralambos Stamatis

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Kondrat, S., von Lieres, E. (2022). Mechanisms and Effects of Substrate Channelling in Enzymatic Cascades. In: Stamatis, H. (eds) Multienzymatic Assemblies. Methods in Molecular Biology, vol 2487. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2269-8_3

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