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One-Dimensional STED Microscopy in Optical Tweezers

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Optical Tweezers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2478))

Abstract

Optical tweezers and fluorescence microscopy are powerful methods for investigating the mechanical and structural properties of biomolecules and for studying the dynamics of the biomolecular processes that these molecules are involved in. Here we provide an outline of the concurrent use of optical tweezers and fluorescence microscopy for analyzing biomolecular processes. In particular, we focus on the use of super-resolution microscopy in optical tweezers, which allows visualization of molecules at the higher molecular densities that are typically encountered in living systems. We provide specific details on the alignment procedures of the optical pathways for confocal fluorescence microscopy and 1D-STED microscopy and elaborate on how to diagnose and correct optical aberrations and STED phase plate misalignments.

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Authors and Affiliations

  1. Department of Physics and Astronomy, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands

    Tianlong Man, Joost J. Geldhof, Erwin J. G. Peterman, Gijs J. L. Wuite & Iddo Heller

Authors
  1. Tianlong Man
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  2. Joost J. Geldhof
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  3. Erwin J. G. Peterman
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  4. Gijs J. L. Wuite
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  5. Iddo Heller
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Corresponding author

Correspondence to Iddo Heller .

Editor information

Editors and Affiliations

  1. Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY, USA

    Arne Gennerich

Ethics declarations

The combined optical tweezers and fluorescence technologies used in this article are patented and licensed to LUMICKS B.V., in which I.H, E.J.G.P., and G.J.L.W. declare a financial interest.

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Man, T., Geldhof, J.J., Peterman, E.J.G., Wuite, G.J.L., Heller, I. (2022). One-Dimensional STED Microscopy in Optical Tweezers. In: Gennerich, A. (eds) Optical Tweezers. Methods in Molecular Biology, vol 2478. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2229-2_6

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  • DOI: https://doi.org/10.1007/978-1-0716-2229-2_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2228-5

  • Online ISBN: 978-1-0716-2229-2

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