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RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens

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VEGF Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2475))

Abstract

Different pro-angiogenic factors, such as vascular endothelial growth factor-A (VEGF-A), have been related to microvascular density, clinicopathologic factors, and poor prognosis in many tumors. VEGF-A binds its receptor 2 (VEGFR2) to induce neo-angiogenesis, a constant hallmark of tumor initiation and progression. Based on VEGF-A/VEGFR2 relevance in tumor angiogenesis, several inhibitors were developed. However, the clinical benefits of anti-angiogenic therapies are limited because tumors activate different mechanisms of drug resistance.

The need for understanding tumor biology, limitation or failure of anti-angiogenic therapies, and the demand for a personalized therapeutic approach has boosted the search for robust biomarkers for patient stratification as responder or non-responder to anti-VEGF therapies.

This chapter presents a detailed protocol to perform chromogenic VEGF-A mRNA detection and quantification in human tumor bioptic specimens using RNAscope technology and RNA-in situ hybridization (ISH) algorithm. RNAscope for VEGF-A detection, even for small amounts, is compatible with precious clinical samples and diagnostic laboratory workflows.

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Author information

Authors and Affiliations

  1. Department of Basic Medical Sciences, Neurosciences and Sensory Organs, Section of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy

    Tiziana Annese, Roberto Tamma & Domenico Ribatti

Authors
  1. Tiziana Annese
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  2. Roberto Tamma
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  3. Domenico Ribatti
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Corresponding author

Correspondence to Domenico Ribatti .

Editor information

Editors and Affiliations

  1. Radcliffe Department of Medicine, University of Oxford, Oxford, Oxfordshire, UK

    Lorna R. Fiedler

  2. Department of Comparative Biomedical Sciences, Royal Veterinary College, London, UK

    Caroline Pellet-Many

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Annese, T., Tamma, R., Ribatti, D. (2022). RNAscope for VEGF-A Detection in Human Tumor Bioptic Specimens. In: Fiedler, L.R., Pellet-Many, C. (eds) VEGF Signaling. Methods in Molecular Biology, vol 2475. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2217-9_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2217-9_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2216-2

  • Online ISBN: 978-1-0716-2217-9

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