Abstract
The retention using selective hook (RUSH) system enables us to synchronize and visualize the movement of cargoes along the secretory pathway. A fluorescently tagged cargo of interest is retained in the endoplasmic reticulum and released in a biotin-dependent manner. Here, we report a detailed protocol describing the steps necessary to perform RUSH experiments to study secretory granule biogenesis at the trans-Golgi network (TGN).
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Asensio, C.S. (2022). Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH). In: Shen, J. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 2473. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2209-4_3
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DOI: https://doi.org/10.1007/978-1-0716-2209-4_3
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2208-7
Online ISBN: 978-1-0716-2209-4
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