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Genetic Methods for Cellular Manipulation in C. elegans

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C. elegans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2468))

Abstract

Neuron manipulation in vivo by ablation, activation or inactivation, and regulation of gene expression is essential for dissecting nervous system function. Here we describe genetic means for neuron manipulation in the nematode C. elegans, and provide protocols for generating transgenic animals containing these genetic tools.

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Acknowledgments

I would like to thank Shai Shaham, Aakanksha Singhvi, In Hae Lee and Sean Wallace for comments and discussions on this manuscript.

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Authors and Affiliations

  1. Department of Biology, Technion- Israel Institute of Technology, Haifa, Israel

    Menachem Katz

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  1. Menachem Katz
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Correspondence to Menachem Katz .

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  1. Department of Biological Sciences, New Jersey Institute of Technology, Newark, NJ, USA

    Gal Haspel

  2. Department of Neuroscience, Brown University, Providence, RI, USA

    Anne C. Hart

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Katz, M. (2022). Genetic Methods for Cellular Manipulation in C. elegans. In: Haspel, G., Hart, A.C. (eds) C. elegans. Methods in Molecular Biology, vol 2468. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2181-3_4

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  • DOI: https://doi.org/10.1007/978-1-0716-2181-3_4

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