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Recording and Quantifying C. elegans Behavior

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C. elegans

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2468))

Abstract

Studies of C. elegans behavior have been crucial in identifying genetic pathways that control nervous system development and function, as well as basic principles of neural circuit function. Modern analysis of C. elegans behavior commonly relies on video recordings of animals, followed by automated image analysis and behavior quantification. Here, we describe two methods for recording and quantifying C. elegans behavior: a single-worm tracking approach that provides high-resolution behavioral data for individual animals and a multi-worm tracking approach that allows for quantification of the behavior of many animals in parallel. These approaches should be useful to a wide range of researchers studying the nervous system and behavior of C. elegans.

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Author information

Author notes

  1. Navin Pokala and Steven W. Flavell contributed equally to this work.

Authors and Affiliations

  1. Department of Biological and Chemical Sciences, Theobald Science Center, New York Institute of Technology, Old Westbury, NY, USA

    Navin Pokala

  2. Department of Brain & Cognitive Sciences, Picower Institute for Learning & Memory, Massachusetts Institute of Technology, Cambridge, MA, USA

    Steven W. Flavell

Authors
  1. Navin Pokala
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  2. Steven W. Flavell
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Corresponding authors

Correspondence to Navin Pokala or Steven W. Flavell .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, New Jersey Institute of Technology, Newark, NJ, USA

    Gal Haspel

  2. Department of Neuroscience, Brown University, Providence, RI, USA

    Anne C. Hart

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Pokala, N., Flavell, S.W. (2022). Recording and Quantifying C. elegans Behavior. In: Haspel, G., Hart, A.C. (eds) C. elegans. Methods in Molecular Biology, vol 2468. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2181-3_20

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  • DOI: https://doi.org/10.1007/978-1-0716-2181-3_20

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2180-6

  • Online ISBN: 978-1-0716-2181-3

  • eBook Packages: Springer Protocols

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