Abstract
Different parts of a plant can be simultaneously exposed to very different conditions, for example a leaf moving in and out of shadow. In addition to local responses, transmission of information between different tissues and organs is thought to affect the coordination of overall responses to changing environmental conditions. An important adaptive role is played by the stomata, which regulate the evaporation of water vapor and supply of CO2 for photosynthesis. Here, we describe a method to study the effect of distally triggered systemic signals on stomatal conductance. The experimental set up, consisting of a growth chamber and a leaf gas exchange measuring system, enables time-resolved measurements on an intact leaf while maintaining a full control over the environmental conditions of the measured leaf and the whole seedling. The method can be used as a powerful tool to study short- and long-term stomatal responses to changes in different environmental variables, such as light.
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Acknowledgments
We thank Mikael Brosché and Pedro Aphalo for fruitful discussions and critical comments on the manuscript and Tuomas Puukko for providing the pictures. The authors were supported by the Academy of Finland (grants 333703 and 336359 to M.S.), Ella and Georg Ehrnrooth Foundation (M.S.), Finnish Cultural Foundation (M.S.), and Alfred Kordelin Foundation (S.E.).
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Ehonen, S., Sierla, M. (2022). Gas Exchange Measurements in Systemic Signaling Studies. In: Yoshida, T. (eds) Abscisic Acid. Methods in Molecular Biology, vol 2462. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2156-1_9
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DOI: https://doi.org/10.1007/978-1-0716-2156-1_9
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