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Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System

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Glycan Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2460))

Abstract

Glycan microarray is an essential tool to study glycan-binding proteins called lectins. Using glycan microarrays, glycan-binding specificity can be analyzed by incubation with an array in which a series of glycans are immobilized. Various research groups in the world have developed glycan microarray. Among them, our glycan microarray has two unique points: one is the incorporation of the evanescent-field fluorescence detection system, and another is the use of multivalent glycopolymers. These two unique properties allow high-sensitive detection from a relatively limited amount of only nanograms of lectins, which could even be applied in crude samples such as cell lysates and cell culture media. Thus, this system is suitable for the first screening of lectins, lectin-like molecules, lectin candidates, and lectin mutants. Here we describe the protocols to analyze glycan-binding specificity of lectins using our glycan microarray system.

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Authors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Lalhaba Oinam & Hiroaki Tateno

Authors
  1. Lalhaba Oinam
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  2. Hiroaki Tateno
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Corresponding author

Correspondence to Hiroaki Tateno .

Editor information

Editors and Affiliations

  1. Carbohydrate Signalling Group, Discipline of Microbiology School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Michelle Kilcoyne

  2. Advanced Glycoscience Research Cluster, School of Natural Sciences, National University of Ireland Galway, Galway, Ireland

    Jared Q. Gerlach

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Oinam, L., Tateno, H. (2022). Evaluation of Glycan-Binding Specificity by Glycoconjugate Microarray with an Evanescent-Field Fluorescence Detection System. In: Kilcoyne, M., Gerlach, J.Q. (eds) Glycan Microarrays. Methods in Molecular Biology, vol 2460. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2148-6_2

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  • DOI: https://doi.org/10.1007/978-1-0716-2148-6_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2147-9

  • Online ISBN: 978-1-0716-2148-6

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