Abstract
Glycan microarrays are widely used to elucidate carbohydrate binding specificity and affinity of various analytes including proteins, microorganisms, cells, and tissues. Glycan microarrays comprise a wide variety of platforms, differing in surface chemistry, presentation of carbohydrates, carbohydrate valency, and detection strategies, all of which impact on analyte performance. This chapter describes detailed methods for printing neoglycoprotein and glycoprotein microarrays on hydrogel-coated slides and incubation of these glycan microarrays with fluorescently labeled lectins.
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Acknowledgments
MK thanks the Royal Society of Chemistry (RSC) Analytical Chemistry Trust Fund (ACTF) for the 2018 RSC ACTF Fellowship award.
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Gerlach, J.Q., Le Berre, M., Kilcoyne, M. (2022). Neoglycoprotein and Glycoprotein Printing on a Hydrogel Functionalized Microarray Surface and Incubation with Labeled Lectins. In: Kilcoyne, M., Gerlach, J.Q. (eds) Glycan Microarrays. Methods in Molecular Biology, vol 2460. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2148-6_1
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DOI: https://doi.org/10.1007/978-1-0716-2148-6_1
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