Abstract
Numerous cancer models have been developed to investigate the effects of mechanical stress on the biology of cells. Here we describe a protocol to fabricate a perfusion model to culture 3-dimensional (3D) ovarian cancer nodules under constant flow. The modular design of this model allows for a wide range of treatment regimens and combinations, including PDT and chemotherapy. Finally, methods for a number of readouts are detailed, allowing researchers to investigate a variety of biological and cytotoxic parameters related to mechanical stress and therapeutic modalities.
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Acknowledgments
This work was supported by the National Institute of Health grants R00CA175292 (to IR) and R01CA158415 (to TH).
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Nath, S., Pigula, M., Hasan, T., Rizvi, I. (2022). A Perfusion Model to Evaluate Response to Photodynamic Therapy in 3D Tumors. In: Broekgaarden, M., Zhang, H., Korbelik, M., Hamblin, M.R., Heger, M. (eds) Photodynamic Therapy. Methods in Molecular Biology, vol 2451. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2099-1_4
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DOI: https://doi.org/10.1007/978-1-0716-2099-1_4
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