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Subcutaneous Xenograft Models for Studying PDT In Vivo

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Photodynamic Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2451))

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Abstract

The most facile, reproducible, and robust in vivo models for evaluating the anticancer efficacy of photodynamic therapy (PDT) are subcutaneous xenograft models of human tumors. The accessibility and practicality of light irradiation protocols for treating subcutaneous xenograft models also increase their value as relatively rapid tools to expedite the testing of novel photosensitizers, respective formulations, and treatment regimens for PDT. This chapter summarizes the methods used in the literature to prepare various types of subcutaneous xenograft models of human cancers and syngeneic models to explore the role of PDT in immuno-oncology. This chapter also summarizes the PDT treatment protocols tested on the subcutaneous models, and the procedures used to evaluate the efficacy at the molecular, macromolecular, and host organism levels.

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Acknowledgments

We would like to acknowledge NIH grants K99CA215301 and R00CA215301 to Girgis Obaid, and P01CA084203 and S10 ODO1232601 to Tayyaba Hasan.

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Authors and Affiliations

  1. Wellman Center for Photomedicine, Department of Dermatology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

    Girgis Obaid & Tayyaba Hasan

Authors
  1. Girgis Obaid
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  2. Tayyaba Hasan
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Corresponding author

Correspondence to Girgis Obaid .

Editor information

Editors and Affiliations

  1. Institute for Advanced Biosciences, Université de Grenoble Alpes, Grenoble, France

    Mans Broekgaarden

  2. van ‘t Hoff Institute for Molecular Sciences, University of Amsterdam, Amsterdam, The Netherlands

    Hong Zhang

  3. British Columbia Cancer Research Centre, Vancouver, BC, Canada

    Mladen Korbelik

  4. Laser Research Centre, University of Johannesburg, Doornfontein, South Africa

    Michael R. Hamblin

  5. Jiaxing Key Laboratory for Photonanomedicine and Experimental Therapeutics, Department of Pharmaceutics, Jiaxing University College of Medicine, Jiaxing, Zhejiang, China

    Michal Heger

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Obaid, G., Hasan, T. (2022). Subcutaneous Xenograft Models for Studying PDT In Vivo. In: Broekgaarden, M., Zhang, H., Korbelik, M., Hamblin, M.R., Heger, M. (eds) Photodynamic Therapy. Methods in Molecular Biology, vol 2451. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2099-1_10

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  • DOI: https://doi.org/10.1007/978-1-0716-2099-1_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2098-4

  • Online ISBN: 978-1-0716-2099-1

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