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Methods for Assessing the Effects of Galectins on Leukocyte Trafficking and Clearance

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Galectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2442))

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Abstract

Numerous protocols exist for investigating leukocyte recruitment and clearance both in vitro and in vivo. Here we describe an in vitro flow chamber assay typically used for studying the mechanisms underpinning leukocyte movement through the endothelium and zymosan-induced peritonitis, an acute in vivo model of inflammation that enables both leukocyte trafficking and clearance to be monitored. Insight is given as to how these models can be used to study the actions of galectins on the inflammatory process.

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Authors and Affiliations

  1. William Harvey Research Institute, Barts and The London School of Medicine, Queen Mary University of London, London, UK

    Hannah L. Law & Dianne Cooper

Authors
  1. Hannah L. Law
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  2. Dianne Cooper
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Corresponding author

Correspondence to Dianne Cooper .

Editor information

Editors and Affiliations

  1. Joint Program in Transfusion Medicine Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA

    Sean R. Stowell

  2. Joint Program in Transfusion Medicine Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA

    Connie M. Arthur

  3. Department of Surgery Beth Israel Deaconess Medical Center National Center for Functional Glycomics, Harvard Medical School, Boston, MA, USA

    Richard D. Cummings

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Law, H.L., Cooper, D. (2022). Methods for Assessing the Effects of Galectins on Leukocyte Trafficking and Clearance. In: Stowell, S.R., Arthur, C.M., Cummings, R.D. (eds) Galectins. Methods in Molecular Biology, vol 2442. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2055-7_31

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  • DOI: https://doi.org/10.1007/978-1-0716-2055-7_31

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2054-0

  • Online ISBN: 978-1-0716-2055-7

  • eBook Packages: Springer Protocols

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