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Fluorescent Microscopy pp 197–209Cite as

Hydrophobic and Hydrogel-Based Methods for Passive Tissue Clearing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2440))

Abstract

Optical tissue clearing enables the precise imaging of cellular and subcellular structures in whole organs and tissues without the need for physical tissue sectioning. By combining tissue clearing with confocal or lightsheet microscopy, 3D images can be generated of entire specimens for visualization and large-scale data analysis. Here we demonstrate two different passive tissue clearing techniques that are compatible with immunofluorescent staining and lightsheet microscopy: PACT, an aqueous hydrogel–based clearing method, and iDISCO+, an organic solvent–based clearing method.

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Acknowledgments

This work was funded by Mission Connect, a program of TIRR Foundation. Use of the Texas A&M Microscopy and Imaging Center is acknowledged.

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Authors and Affiliations

  1. Department of Biology, Texas A&M University, College Station, TX, USA

    Frank L. Jalufka, Sun Won Min, Madison E. Platt, Anna L. Pritchard, Theodore E. Margo, Alexandra O. Vernino, Megan A. Kirchhoff & Dylan A. McCreedy

  2. Texas A&M Institute for Neuroscience (TAMIN), Texas A&M University, College Station, TX, USA

    Ryan T. Massopust & Dylan A. McCreedy

Authors
  1. Frank L. Jalufka
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  2. Sun Won Min
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  3. Madison E. Platt
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  4. Anna L. Pritchard
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  5. Theodore E. Margo
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  6. Alexandra O. Vernino
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  7. Megan A. Kirchhoff
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  8. Ryan T. Massopust
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  9. Dylan A. McCreedy
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Corresponding author

Correspondence to Dylan A. McCreedy .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Center for Human Immunology, The University of Western Ontario, London, ON, Canada

    Bryan Heit

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Jalufka, F.L. et al. (2022). Hydrophobic and Hydrogel-Based Methods for Passive Tissue Clearing. In: Heit, B. (eds) Fluorescent Microscopy. Methods in Molecular Biology, vol 2440. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2051-9_12

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  • DOI: https://doi.org/10.1007/978-1-0716-2051-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2050-2

  • Online ISBN: 978-1-0716-2051-9

  • eBook Packages: Springer Protocols

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