Abstract
Disease outbreaks in swine production cause severe economic loss. Conventional diagnostic methods are laborious and less sensitive or time consuming. Recent molecular methods of diagnosis of diseases especially genome detection play a major role in the diagnosis and control of diseases. Although several molecular diagnostic techniques are available, still not all the techniques are employable to detect different pathogens simultaneously. Most of the recent outbreaks are involved with more than one pathogen. Hence, a simple and rapid technique which can detect different targets/pathogens simultaneously is in need of time. LDR-FMA is such an assay where multiplexing is possible. Although LDR-FMA is mainly used to detect single nucleotide polymorphism, this technique still could be adapted for simultaneous detection of different pathogens.
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Raja, A. (2022). Ligase Detection Reaction-Fluorescent Microsphere Assay. In: Deb, R., Yadav, A.K., Rajkhowa, S., Malik, Y.S. (eds) Protocols for the Diagnosis of Pig Viral Diseases. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2043-4_21
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DOI: https://doi.org/10.1007/978-1-0716-2043-4_21
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