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Optogenetics in Complex Model Systems (Non-Human Primate)

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Measuring Cerebellar Function

Part of the book series: Neuromethods ((NM,volume 177))

Abstract

Primates use rapid eye movements, called saccades, to scan their surroundings. Most of the well-studied neuronal elements that generate saccades are located in the brainstem, but recently our labs and others showed that the midline cerebellum is required for the production of accurate and stereotypical saccades. This oculomotor cerebellum receives mossy fiber inputs from saccade-related areas in the brainstem and sends its outputs to the brainstem saccade burst generator. How Purkinje cell simple spike activity is formed, and how the activity affects the movement in real-time are not well understood. In this chapter, we describe techniques to address these questions. Using optogenetics we manipulate the simple spike activity of cerebellar Purkinje cells while the saccade is ongoing. We also express optogenetic opsin to inhibit a subset of mossy fiber inputs to the cerebellum and examine the effects of the inhibition on simple spike activity.

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Acknowledgments

Our studies described here were supported by National Institute of Health grants: EY028902 (RS), EY023277 (YK), OD010425, RR00166 (Washington National Primate Research Center), P30EY001730 (Vision Research Core of UW), and National Science Foundation BCS-1724176 (RS, YK).

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Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Washington, Seattle, WA, USA

    Robijanto Soetedjo

  2. Washington National Primate Research Center, University of Washington, Seattle, WA, USA

    Robijanto Soetedjo & Yoshiko Kojima

  3. Department of Otolaryngology-Head and Neck Surgery, University of Washington, Seattle, WA, USA

    Yoshiko Kojima

Authors
  1. Robijanto Soetedjo
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  2. Yoshiko Kojima
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Correspondence to Robijanto Soetedjo .

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Editors and Affiliations

  1. Houston, TX, USA

    Roy V. Sillitoe

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Soetedjo, R., Kojima, Y. (2022). Optogenetics in Complex Model Systems (Non-Human Primate). In: Sillitoe, R.V. (eds) Measuring Cerebellar Function. Neuromethods, vol 177. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2026-7_15

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  • DOI: https://doi.org/10.1007/978-1-0716-2026-7_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2025-0

  • Online ISBN: 978-1-0716-2026-7

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