Abstract
In response to treating heavy metal pollution in contaminated sites, screening of endophytic fungi against heavy metal offers a promising bioremediation approach. It is important to investigate the potential of endophytic fungal communities, to be applied as biosorption materials known as “biosorbents” for the removal of heavy metal ions in aqueous solution. In this chapter, we describe a process of obtaining pure endophytic fungal cultures from plants of interest and screening for their tolerance against heavy metal ions. These procedures are carried out using the conventional agar-plate-based method following standard aseptic techniques.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Guo B, Wang Y, Sun X, Tang K (2008) Bioactive natural products from endophytes: a review. Appl Biochem Microbiol 44:136–142. https://doi.org/10.1134/S0003683808020026
Jalgaonwala RE, Mohite BV, Mahajan RT (2011) A review: natural products from plant associated endophytic fungi. J Microbiol Biotech Res 1:21–32
Lu H, Zou WX, Meng JC et al (2000) New bioactive metabolites produced by Colletotrichum sp., an endophytic fungus in Artemisia annua. Plant Sci 151:67–73. https://doi.org/10.1016/S0168-9452(99)00199-5
Rehman S, Shawl AS, Verma V et al (2008) An endophytic Neurospora sp. from Nothapodytes foetida producing camptothecin. Prikl Biokhim Mikrobiol 44:225–231. https://doi.org/10.1134/s0003683808020130
Strobel G, Ford E, Worapong J et al (2002) Isopestacin, an isobenzofuranone from Pestalotiopsis microspora, possessing antifungal and antioxidant activities. Phytochemistry 60:179–183. https://doi.org/10.1016/S0031-9422(02)00062-6
Strobel G, Daisy B (2003) Bioprospecting for microbial Endophytes and their natural products. Microbiol Mol Biol Rev 67:491–502. https://doi.org/10.1128/mmbr.67.4.491-502.2003
Wiyakrutta S, Sriubolmas N, Panphut W et al (2004) Endophytic fungi with anti-microbial, anti-cancer and anti-malarial activities isolated from Thai medicinal plants. World J Microbiol Biotechnol 20:265–272. https://doi.org/10.1023/B:WIBI.0000023832.27679.a8
Bapat PM, Kundu S, Wangikar PP (2003) An optimized method for Aspergillus niger spore production on natural carrier substrates. Biotechnol Prog 19:1683–1688. https://doi.org/10.1021/bp0341141
Gadd GM (1994) Interactions of fungi with toxic metals. In: Powell KA, Renwick A, Peberdy JF (eds) The genus Aspergillus: from taxonomy and genetics to industrial application. Springer US, Boston, pp 361–374
Svecova L, Spanelova M, Kubal M, Guibal E (2006) Cadmium, lead and mercury biosorption on waste fungal biomass issued from fermentation industry. I. Equilibrium studies. Sep Purif Technol 52:142–153. https://doi.org/10.1016/j.seppur.2006.03.024
Wang J, Chen C (2009) Biosorbents for heavy metals removal and their future. Biotechnol Adv 27:195–226. https://doi.org/10.1016/j.biotechadv.2008.11.002
Siddiqui E, Pandey J (2019) Assessment of heavy metal pollution in water and surface sediment and evaluation of ecological risks associated with sediment contamination in the Ganga River: a basin-scale study. Environ Sci Pollut Res 26:10926–10940. https://doi.org/10.1007/s11356-019-04495-6
Taghinia Hejabi A, Basavarajappa HT, Karbassi AR, Monavari SM (2011) Heavy metal pollution in water and sediments in the Kabini River, Karnataka, India. Environ Monit Assess 182:1–13. https://doi.org/10.1007/s10661-010-1854-0
Ramos L, Fernández MA, González MJ, Hernández LM (1999) Heavy metal pollution in water, sediments, and earthworms from the Ebro River, Spain. Bull Environ Contam Toxicol 63:305–311. https://doi.org/10.1007/s001289900981
Cheng S (2003) Heavy metal pollution in China: origin, pattern and control. Environ Sci Pollut Res 10:192–198. https://doi.org/10.1065/espr2002.11.141.1
Gafur NA, Sakakibara M, Sano S, Sera K (2018) A case study of heavy metal pollution in water of Bone river by artisanal small-scale gold mine activities in eastern part of Gorontalo, Indonesia. Water (Switzerland) 10:1–10. https://doi.org/10.3390/w10111507
Begum A, Ramaiah M, Harikrishna et al (2009) Heavy metal pollution and chemical profile of Cauvery river water. J Chem 6:47–52. https://doi.org/10.1155/2009/154610
Choo J, Sabri NBM, Tan D. et al (2015) Heavy metal resistant endophytic fungi isolated from Nypa fruticans in Kuching Wetland National Park. Ocean Sci J 50:445–453. https://doi.org/10.1007/s12601-015-0040-2
Kjer J, Debbab A, Aly AH, Proksch P (2010) Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. Nat Protoc 5:479–490. https://doi.org/10.1038/nprot.2009.233
Guo LD, Hyde KD, Liew ECY (2000) Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences. New Phytol 147:617–630. https://doi.org/10.1046/j.1469-8137.2000.00716.x
Photita W, Lumyong S, Lumyong P et al (2001) Fungi on Musa acuminata in Hong Kong. Water Resour Manag 14:99–106
Ruriani E, Sunarti TC, Meryandini A (2012) Yeast isolation for bioethanol production. Hayati J Biosci 19:145–149. https://doi.org/10.4308/hjb.19.3.145
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Choo, J., Wong, C., Müller, M. (2022). Protocol for Screening Endophytic Fungi Against Heavy Metals. In: Udayanga, D., Bhatt, P., Manamgoda, D., Saez, J.M. (eds) Mycoremediation Protocols. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2006-9_5
Download citation
DOI: https://doi.org/10.1007/978-1-0716-2006-9_5
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2005-2
Online ISBN: 978-1-0716-2006-9
eBook Packages: Springer Protocols