Abstract
Today, water body pollution is one of the world’s biggest environmental problems. In this sense, there is a high demand for the design and potential development of new devices for the detection and quantification of pollutants present in the environment. Biosensors are ideal for detecting and measuring environmental pollution reliably, specifically, and sensitively. Among them, electrochemical enzyme biosensors have many advantages such as their high selectivity and sensitivity to the target substrate, which is presented as an opportunity to significantly improve the diagnosis and monitoring of environmental pollutants. The protocol presented in this chapter consists of a technique for monitoring contaminants in surface waters using the enzyme laccase of fungal origin in the formation of an enzyme biosensor.
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References
Claude D, Houssemeddine G, Andriy B, Jean-Marc C (2007) Whole-cell algal biosensors for urban waters monitoring. Ther Nova 7:1507–1514
Nigam VK, Shukla P (2015) Enzyme-based biosensors for detection of environmental pollutants-a review. J Microbiol Biotechnol 25(11):1773–1781
Colborn T, Myers JP, Dumanoski D (1997) Nuestro futuro robado. Ecoespaña, Madrid
Songa EA, Arotiba OA, Owino JH, Jahed N, Baker PG, Iwuoha EI (2009) Electrochemical detection of glyphosate herbicide using horseradish peroxidase immobilized on sulfonated polymer matrix. Bioelectrochemistry 75(2):117–123
Vorčáková K, Štěpánková Š, Sedlák M, Vytřas K (2015) Electrochemical sensors for the estimation of the inhibitory effect of phenylcarbamates to cholinesterase. Chemosensors 3(4):274–283
Wang X, Yao MQ, Liu L, Cao Y, Bao J (2016) Degradation of chlorpyrifos in contaminated soil by immobilized laccase. J Serb Chem Soc 81(10):1215–1224
Liu T, Su H, Qu X, Ju P, Cui L, Ai S (2011) Acetylcholinesterase biosensor based on 3-carboxyphenylboronic acid/reduced graphene oxide–gold nanocomposites modified electrode for amperometric detection of organophosphorus and carbamate pesticides. Sens Actuators B Chem 160(1):1255–1261
Songa EA, Okonkwo JO (2016) Recent approaches to improving selectivity and sensitivity of enzyme-based biosensors for organophosphorus pesticides: a review. Talanta 155:289–304
Cosnier S (2003) Biosensors based on electropolymerized films: new trends. Anal Bioanal Chem 377(3):507–520
Riu J, Maroto A, Rius FX (2006) Nanosensors in environmental analysis. Talanta 69(2):288–301
Thurston CF (1994) The structure and function of fungal laccases. Microbiology 140(1):19–26
Gianfreda L, Xu F, Bollag JM (1999) Laccases: a useful group of oxidoreductive enzymes. Biorem J 3(1):1–26
Diamantidis G, Effosse A, Potier P, Bally R (2000) Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum. Soil Biol Biochem 32(7):919–927
Dean JF, Eriksson KEL (1994) Laccase and the deposition of lignin in vascular plants. Holzforschung 48:21–33
Bajaj M, Gallert C, Winter J (2008) Biodegradation of high phenol containing synthetic wastewater by an aerobic fixed bed reactor. Bioresour Technol 99(17):8376–8381
Bhakta SA, Evans E, Benavidez TE, Garcia CD (2015) Protein adsorption onto nanomaterials for the development of biosensors and analytical devices: a review. Anal Chim Acta 872:7–25
Kudanga T, Nyanhongo GS, Guebitz GM, Burton S (2011) Potential applications of laccase-mediated coupling and grafting reactions: a review. Enzym Microb Technol 48(3):195–208
Montereali MR, Della Seta L, Vastarella W, Pilloton R (2010) A disposable Laccase–Tyrosinase based biosensor for amperometric detection of phenolic compounds in must and wine. J Mol Catal B Enzym 64(3–4):189–194
Nazari M, Kashanian S, Rafipour R (2015) Laccase immobilization on the electrode surface to design a biosensor for the detection of phenolic compounds such as catechol. Spectrochim Acta A Mol Biomol Spectrosc 145:130–138
Portaccio M, Di Tuoro D, Arduini F, Moscone D, Cammarota M, Mita DG, Lepore M (2013) Laccase biosensor based on screen-printed electrode modified with thionine–carbon black nanocomposite, for Bisphenol A detection. Electrochim Acta 109:340–347
Freire RS, Duran N, Wang J, Kubota LT (2002) Laccase-based screen-printed electrode for amperometric detection of phenolic compounds. Anal Lett 35(1):29–38
ElKaoutit M, Naranjo-Rodriguez I, Temsamani KR, Hernández-Artiga MP, Bellido-Milla D, de Cisneros JL (2008) A comparison of three-amperometric phenoloxidase–Sonogel–Carbon based biosensors for determination of polyphenols in beers. Food Chem 110(4):1019–1024
Di Fusco M, Tortolini C, Deriu D, Mazzei F (2010) Laccase-based biosensor for the determination of polyphenol index in wine. Talanta 81(1–2):235–240
Fonseca MI, Shimizu E, Zapata PD, Villalba LL (2010) Copper inducing effect on laccase production of white rot fungi native from Misiones (Argentina). Enzym Microb Technol 46(6):534–539
Sabela MI, Gumede NJ, Singh P, Bisetty K (2012) Evaluation of antioxidants in herbal tea with a Laccase biosensor. Int J Electrochem Sci 7(6):4918–4928
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Schimpf, A.R.A., Rodríguez, D., Fonseca, M.I., Zapata, P.D. (2022). Enzyme Biosensors for the Detection of Environmental Contaminants. In: Udayanga, D., Bhatt, P., Manamgoda, D., Saez, J.M. (eds) Mycoremediation Protocols. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2006-9_21
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DOI: https://doi.org/10.1007/978-1-0716-2006-9_21
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