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ER+ Breast Cancer Mammosphere Formation and Analysis

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Immunohistochemistry and Immunocytochemistry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2422))

Abstract

Mammosphere formation assays are a popular and convenient technique in the study of breast cancer by providing an in vitro mechanism by which to study breast cancer stem cell (BCSC) contribution to tumorigenesis, as well as more closely mimicking the three-dimensional tumor microenvironment. In these assays, BCSCs are stimulated to proliferate in low adherence tissue culture dishes and the resulting mammospheres exhibit activation of stem cell-related signaling pathways. Here we describe the process for generating and analyzing mammospheres under varying conditions.

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Authors and Affiliations

  1. Department of Genetics, Louisiana State University Health Sciences Center, New Orleans, LA, USA

    Ciera S. Singleton & Judy S. Crabtree

  2. Department of Advanced Technology R&D, Nexcelom Bioscience LLC, Lawrence, MA, USA

    Leo Li-Ying Chan, Kelsey J. McCulley & Sarah L. Kessel

  3. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA

    Luis Del Valle

Authors
  1. Ciera S. Singleton
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  2. Leo Li-Ying Chan
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  3. Kelsey J. McCulley
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  4. Sarah L. Kessel
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  5. Luis Del Valle
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  6. Judy S. Crabtree
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Corresponding author

Correspondence to Judy S. Crabtree .

Editor information

Editors and Affiliations

  1. Department of Pathology and Medicine & Louisiana Cancer Research Center, Louisiana State University Health Sciences Center, New Orleans, LA, USA

    Luis Del Valle

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Singleton, C.S., Chan, L.LY., McCulley, K.J., Kessel, S.L., Del Valle, L., Crabtree, J.S. (2022). ER+ Breast Cancer Mammosphere Formation and Analysis. In: Del Valle, L. (eds) Immunohistochemistry and Immunocytochemistry. Methods in Molecular Biology, vol 2422. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1948-3_16

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  • DOI: https://doi.org/10.1007/978-1-0716-1948-3_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1947-6

  • Online ISBN: 978-1-0716-1948-3

  • eBook Packages: Springer Protocols

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