Abstract
One-carbon metabolism (1CM) plays a central role in liver physiology, being the source of essential metabolites such as S-adenosylmethionine, the main alkylating agent in living cells, and glutathione, their most important nonenzymatic antioxidant defense. Impairment of 1CM in hepatocytes is a recognized factor associated to chronic liver disorders and hepatocellular carcinoma. With this in mind, we have proposed the concept of functional biomarker referring to a cellular pathway that can be systematically monitored as indicative of a particular physiological or pathological condition. Here we describe a targeted mass spectrometry (MRM) protocol to simultaneously quantify 13 1CM enzymes in liver tissue specimens.
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Acknowledgments
This work was supported by the Carlos III Health Institute of Spain—Fondos FEDER (EU); grant PT17/0019/0001 (FJC); Comunidad de Madrid Grant B2017/BMD-3817 (FJC); CSIC PIE Grant 202020E108. CNB-CSIC has been designed Centre of Excellence “Severo Ochoa” and is funded by the Spanish Government through grant SEV2017-0712.
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Guerrero, L., Paradela, A., Corrales, F.J. (2022). Development of a Standardized MRM Method for the Quantification of One Carbon Metabolism Enzymes. In: Corrales, F.J., Paradela, A., Marcilla, M. (eds) Clinical Proteomics. Methods in Molecular Biology, vol 2420. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1936-0_13
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DOI: https://doi.org/10.1007/978-1-0716-1936-0_13
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