Abstract
Protozoan parasites constitute a neglected group of foodborne pathogens for which few validated detection methods are available in shellfish . This chapter describes a nested PCR assay for the detection of Toxoplasma gondii and Cyclospora cayetanensis that was validated in different tissues of oysters . The assay can consistently amplify DNA from as few as 5–10 oocysts in hemolymph or whole-tissue homogenates.
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References
DeWaal CS, Glassman M (2014) Outbreak alert! 2014: a review of foodborne illness in America from 2002–2011. Center for Science in the public interest
CDC (2019) Surveillance for foodborne disease outbreaks, United States, 2017, Annual report. U.S. Department of Health and Human Services, Atlanta, GA
WHO (2010) In: Rees G et al (eds) Safe management of shellfish and harvest waters. IWA Publishing, London
Bahia-Oliveira L, Gomez-Marin J, Shapiro K (2017) Toxoplasma gondii. In: Rose JB, Jiménez-Cisneros B (eds) Global water pathogen project. Michigan State University, East Lansing, MI, UNESCO
Chacin-Bonilla L (2017) Cyclospora Cayetanensis. In: Rose JB, Jiménez-Cisneros B (eds) Global water pathogen project. Michigan State University, East Lansing, MI, UNESCO
Montoya JG, Liesenfeld O (2004) Toxoplasmosis. Lancet 363(9425):1965–1976
Hohweyer J et al (2013) Tools and methods for detecting and characterizing Giardia, Cryptosporidium, and Toxoplasma parasites in marine mollusks. J Food Prot 76(9):1649–1657
Robertson LJ, Gjerde B (2008) Development and use of a pepsin digestion method for analysis of shellfish for Cryptosporidium oocysts and Giardia cysts. J Food Prot 71(5):959–966
Shapiro K et al (2019) Simultaneous detection of four protozoan parasites on leafy greens using a novel multiplex PCR assay. Food Microbiol 84:103252
Willis JE et al (2014) Bioaccumulation and elimination of Cryptosporidium parvum oocysts in experimentally exposed Eastern oysters (Crassostrea virginica) held in static tank aquaria. Int J Food Microbiol 173:72–80
Rousseau A et al (2019) Toxoplasma gondii oocyst infectivity assessed using a sporocyst-based cell culture assay combined with quantitative PCR for environmental applications. Appl Environ Microbiol 85(20):e01189-19
Géba E et al (2020) Use of the bivalve Dreissena polymorpha as a biomonitoring tool to reflect the protozoan load in freshwater bodies. Water Res 170:115297
Manore AJW et al (2019) Comparison of freeze-thaw cycles for nucleic acid extraction and molecular detection of Cryptosporidium parvum and Toxoplasma gondii oocysts in environmental matrices. J Microbiol Methods 156:1–4
Acknowledgments
Development of the nested PCR assay was supported by the Center for Produce Safety and the California Department of Food and Agriculture. Validation of the nested PCR for the detection of protozoan pathogens in oysters was supported by the National Institute of Food and Agriculture, the United States Department of Agriculture.
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Kim, M., Shapiro, K. (2021). Detection of Toxoplasma Gondii and Cyclospora Cayetanensis in Oysters. In: Magnani, M. (eds) Detection and Enumeration of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1932-2_21
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DOI: https://doi.org/10.1007/978-1-0716-1932-2_21
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