Abstract
Atherosclerotic plaques are highly diverse and heterogeneous structures, even within the same individual, and can vary depending on its anatomical location within the vascular bed. Early in the disease and throughout its progression, immune cells infiltrate the lesion, contributing to the plaque phenotype via different mechanisms. Detailed characterization of constituent cell populations within plaques is hence required for more accurate assessment of disease severity and inflammatory burden. A wide range of fluorophore-conjugated antibodies targeted to key cell types implicated in all stages of the disease are commercially available, enabling visualization of the dynamic cellular landscape present within lesions. This chapter describes the use of immunofluorescence staining of atherosclerotic plaque sections to study plaque cellularity and expression of key markers.
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References
Nielsen SH, Jonasson L, Kalogeropoulos K et al (2020) Exploring the role of extracellular matrix proteins to develop biomarkers of plaque vulnerability and outcome. J Intern Med 287(5):493–513
Chan Y-H, Ramji DP (2020) A perspective on targeting inflammation and cytokine actions in atherosclerosis. Future Med Chem 12(7):613–626
Adiguzel E, Ahmad PJ, Franco C et al (2009) Collagens in the progression and complications of atherosclerosis. Vasc Med 14:73–89
Moss JWE, Ramji DP (2016) Cytokines: roles in atherosclerosis disease progression and potential therapeutic targets. Future Med Chem 8(11):1317–1330
Wagsater D, Zhu C, Bjorkegren J et al (2011) MMP-2 and MMP-9 are prominent matrix metalloproteinases during atherosclerosis development in the Ldlr−/−Apob100/100 mouse. Int J Mol Med 28:247–253
Bergheanu SC, Bodde MC, Jukema JW (2017) Pathophysiology and treatment of atherosclerosis: current view and future perspective on lipoprotein modification treatment. Neth Heart J 25(4):231–242
Libby P, Buring JE, Badimon L et al (2019) Atherosclerosis. Nat Rev Dis Primers 5(56):1–18
Moss JWE, Williams JO, Ramji DP (2018) Nutraceuticals as therapeutic agents for atherosclerosis. Biochim Biophys Acta Mol Basis Dis 1864:1562–1572
Im K, Mareninov S, Diaz MFP et al (2019) An introduction to performing immunofluorescence staining. Methods Mol Biol 1897:299–311
Acknowledgements
We thank the British Heart Foundation for financial support (grants PG/16/25/32097 and FS/17/75/33257). AA and RA received PhD studentships from the Kingdom of Saudi Arabia (Jeddah University).
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Chan, YH., Alotibi, R., Alahmadi, A., Ramji, D.P. (2022). Monitoring Cellularity and Expression of Key Markers in Atherosclerotic Plaques. In: Ramji, D. (eds) Atherosclerosis. Methods in Molecular Biology, vol 2419. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1924-7_30
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DOI: https://doi.org/10.1007/978-1-0716-1924-7_30
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1923-0
Online ISBN: 978-1-0716-1924-7
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