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Monitoring Leukocyte Migration During Atherosclerosis In Vivo

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Atherosclerosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2419))

Abstract

Transendothelial leukocyte migration is an early event in the progression of vascular inflammation, the underlying molecular mechanism of atherosclerosis. Inflammatory mediators such as adhesion molecules and chemokines are essential in this process. Leukocyte migration into the vascular wall can be monitored by the detection of CD11b-positive immune cells in animal models of atherosclerosis. This chapter will describe an immunohistochemical technique used to evaluate leukocyte migration in vivo.

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References

  1. Takeda Y, Matoba K, Kawanami D et al (2019) ROCK2 regulates monocyte migration and cell to cell adhesion in vascular endothelial cells. Int J Mol Sci 20:1331

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Acknowledgments

This work was supported by JSPS KAKENHI (20K08645), Suzuken Memorial Foundation, Takeda Science Foundation, MSD Life Science Foundation, and Japan Diabetes Foundation to K.M.

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Authors and Affiliations

  1. Division of Diabetes, Endocrinology and Metabolism, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan

    Keiichiro Matoba

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  1. Keiichiro Matoba
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Correspondence to Keiichiro Matoba .

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Editors and Affiliations

  1. School of Biosciences, Cardiff University, Cardiff, UK

    Dipak Ramji

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Matoba, K. (2022). Monitoring Leukocyte Migration During Atherosclerosis In Vivo. In: Ramji, D. (eds) Atherosclerosis. Methods in Molecular Biology, vol 2419. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1924-7_28

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  • DOI: https://doi.org/10.1007/978-1-0716-1924-7_28

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1923-0

  • Online ISBN: 978-1-0716-1924-7

  • eBook Packages: Springer Protocols

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